Show GC Bases 
Highlight Deoxyribose 
Highlight Phosphate 
2 Nucleotide Pairs 
3 Nucleotide Pairs 
Major and Minor Grooves 
 Major Groove - Red
 Minor Groove - Green

Always begin the animation using the "Show GC Bases" button. Thereafter any of the remaining 5 buttons can be selected. After selecting "Major and Minor Grooves", use the reload button on the main menu bar to bring the DNA molecue back to standard orientation. After doing a reload, you must begin again with "Show GC Bases".